Nehomologni izofunkcijski enzim

Nehomologni izofunkcijski enzim (NISE) je po funkciji analog poznatog enzima, ali imaju jedinstvenu proteinaki sekvencu ili savijanje molekula.^[1]. Kao takav, ne može stvarati zabunu u pogledu EC broja u sistemu Enzima komisije.^[2] Nedavni primjer je enzim urat hidroksilaza (EC 1.14.13.113), koji katalizira razgradnju urata, važan sastojak ciklizaciju dušika. Novootkriveni HpyO gen je nehomolog u odnosu na originalnu gen HpxO, ali još uvijek dijele određene karakteristike koje omogućavaju da obavljaju sličnu reakciju.^[3]

Također pogledajte uredi

Enzim

Reference uredi

^ Marina V. Omelchenko, Michael Y . Galperin, Yuri I Wolf, Eugene V Koonin. (2010): Non-homologous isofunctional enzymes: A systematic analysis of alternative solutions in enzyme evolution. Biology Direct, 5 (31).
^ Seán E. O’Leary, Katherine A. Hicks, Steven E. Ealick, and Tadhg P. Begley. (2009). Biochemical Characterization of the HpxO Enzyme from Klebsiella pneumoniae, a Novel FAD–Dependent Urate Oxidase. Biochemistry. 2009 April 14; 48(14): 3033–3035. doi:10.1021/bi900160b.
^ Magalie Michiel, Nadia Perchat, Alain Perret, Sabine Tricot, Aude Papeil, Marielle Besnard, Véronique de Berardinis, Marcel Salanoubat, and Cécile Fischer. (2012). Microbial urate catabolism: characterization of HpyO, a non-homologous isofunctional isoform of the flavoprotein urate hydroxylase HpxO. Environmental Microbiology reports 4 (6): 642–647. doi:10.1111/j.1758-2229.2012.00390.x. PMID 23760935.

[1] Marina V. Omelchenko, Michael Y . Galperin, Yuri I Wolf, Eugene V Koonin. (2010): Non-homologous isofunctional enzymes: A systematic analysis of alternative solutions in enzyme evolution. Biology Direct, 5 (31).

[2] Seán E. O’Leary, Katherine A. Hicks, Steven E. Ealick, and Tadhg P. Begley. (2009). Biochemical Characterization of the HpxO Enzyme from Klebsiella pneumoniae, a Novel FAD–Dependent Urate Oxidase. Biochemistry. 2009 April 14; 48(14): 3033–3035. doi:10.1021/bi900160b.

[3] Magalie Michiel, Nadia Perchat, Alain Perret, Sabine Tricot, Aude Papeil, Marielle Besnard, Véronique de Berardinis, Marcel Salanoubat, and Cécile Fischer. (2012). Microbial urate catabolism: characterization of HpyO, a non-homologous isofunctional isoform of the flavoprotein urate hydroxylase HpxO. Environmental Microbiology reports 4 (6): 642–647. doi:10.1111/j.1758-2229.2012.00390.x. PMID 23760935.

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