Proteini F-kutije

F-kutijski linkerski domen
F-kutijski linkerski domen
	Struktura LRR linkerskog domena Skp2 u kompleksu Skp1-Skp2.
Identifikatori
Simbol	F-box

Proteini F-kutije su proteini koji sadrže najmanje jedan F-kutijski domen. Prvi identificirani protein F-box jedna je od tri komponente SCF-kompleksa, koji posreduje ubikvitinaciju proteina ciljanih na razgradnju proteasoma 26S.

Sržne komponente

Domen F-kutije je protein strukturnog motiva od oko 50 aminokiselina koji posreduje u interakcijama protein-protein . Ima konsenzusnu sekvencu i varira na nekoliko pozicija. Prvi put je identificiran u ciklinu F.^[2] Motiv F-kutije Skp2, koji se sastoji od tri alfa-heliksa, direktno stupa u interakciju sa proteinom SCF Skp1.^[3] F-kutijski domeni obično postoje u proteinima zajedno s drugim motivima interakcije protein-protein, kao što je ponavljanje bogato leucinom (ilustrirano na slici) i WD-ponavljanje, za koje se smatra da posreduju u interakcijama sa SCF podlogama.^[4]

Funkcija

Proteini F-kutije su također povezani sa ćelijskim funkcijama kao što su transdukcija signala i regulacija ćelijskog ciklusa.^[5] U biljkama su mnogi proteini F-kutije zastupljeni u genskoj mreži koja je široko regulirana mikroRNK-posredovanim utišavanjem gena putem interferirajuće RNK.^[6] Proteini F-kutije uključeni su u rast i razvoj mnogih vegetativnih i reprodukcijskih biljnih organa. Naprimjer, protein F-boks-FOA1 uključen je u signalizaciju apscisinske kiseline (ABA) da utiče na klijanje sjemena.^[7] ACRE189/ACIF1 može regulirati ćelijsku smrt i odbranu kada se prepozna patogen u biljkama duhana i paradajza.^[8]

U ljudskim ćelijama, pod uslovima sa visokim sadržajem gvožđa, dva atoma gvožđa stabilizuju F-kutijski FBXL5, a zatim kompleks posreduje u sveprisutnosti IRP2.^[9]

Regulacija

Nivoi F-kutijskih proteina mogu se regulirati različitim mehanizmima. Do regulacije može doći putem procesa razgradnje proteina i povezivanjem sa SCF kompleksom. Naprimjer, u kvascima se protein F-box Met30 može ubikvitinirati na način ovisan o kullinu.^[10]^[11]

Reference

^ Schulman BA, Carrano AC, Jeffrey PD, et al. (novembar 2000). "Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex". Nature. 408 (6810): 381–6. Bibcode:2000Natur.408..381S. doi:10.1038/35042620. PMID 11099048. S2CID 4300503.
^ Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ. "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box". Cell 86 263-74 1996.
^ Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ (July 1996). "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box". Cell. 86 (2): 263–74. doi:10.1016/S0092-8674(00)80098-7. PubMed.
^ Kipreos ET, Pagano M (2000). "The F-box protein family". Genome Biol. 1 (5): REVIEWS3002. doi:10.1186/gb-2000-1-5-reviews3002. PMC 138887. PMID 11178263.
^ Craig KL, Tyers M (1999). "The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction". Prog. Biophys. Mol. Biol. 72 (3): 299–328. doi:10.1016/S0079-6107(99)00010-3. PMID 10581972.
^ Jones-Rhoades MW, Bartel DP, Bartel B (2006). "MicroRNAS and their regulatory roles in plants". Annu Rev Plant Biol. 57: 19–53. doi:10.1146/annurev.arplant.57.032905.105218. PMID 16669754.
^ Peng, Juan; Yu, Dashi; Wang, Liqun; Xie, Minmin; Yuan, Congying; Wang, Yu; Tang, Dongying; Zhao, Xiaoying; Liu, Xuanming (June 2012). "Arabidopsis F-box gene FOA1 involved in ABA signaling". Science China Life Sciences. 55 (6): 497–506. doi:10.1007/s11427-012-4332-9. ISSN 1869-1889. PMID 22744179.
^ Ha, Van Den Burg; Tsitsigiannis, D. I.; Rowland, O; Lo, J; Rallapalli, G; Maclean, D; Takken, F. L.; Jones, J. D. (2008). "The F-box protein ACRE189/ACIF1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato". Plant Cell. 20 (3): 697.
^ Moroishi, T; Nishiyama, M; Takeda, Y; Iwai, K; Nakayama, K. I. (2011). "The FBXL5-IRP2 axis is integral to control of iron metabolism in vivo". Cell Metabolism. 14 (3): 339.
^ Kaiser, Peter; Su, Ning-Yuan; Yen, James L.; Ouni, Ikram; Flick, Karin (2006-08-08). "The yeast ubiquitin ligase SCFMet30: connecting environmental and intracellular conditions to cell division". Cell Division. 1: 16. doi:10.1186/1747-1028-1-16. ISSN 1747-1028.

Dopunska literatura

Ho M, Tsai P, Chien C (2006). "F-box proteins: the key to protein degradation". J Biomed Sci. 13 (2): 181–91. doi:10.1007/s11373-005-9058-2. PMID 16463014.

Vanjski linkovi

F-Box Proteins na US National Library of Medicine Medical Subject Headings (MeSH)
F-box motifs na US National Library of Medicine Medical Subject Headings (MeSH)

Šablon:Proteini nosači

[pmid11099048-1] Schulman BA, Carrano AC, Jeffrey PD, et al. (novembar 2000). "Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex". Nature. 408 (6810): 381–6. Bibcode:2000Natur.408..381S. doi:10.1038/35042620. PMID 11099048. S2CID 4300503.

[2] Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ. "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box". Cell 86 263-74 1996.

[3] Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ (July 1996). "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box". Cell. 86 (2): 263–74. doi:10.1016/S0092-8674(00)80098-7. PubMed.

[pmid11178263-4] Kipreos ET, Pagano M (2000). "The F-box protein family". Genome Biol. 1 (5): REVIEWS3002. doi:10.1186/gb-2000-1-5-reviews3002. PMC 138887. PMID 11178263.

[pmid10581972-5] Craig KL, Tyers M (1999). "The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction". Prog. Biophys. Mol. Biol. 72 (3): 299–328. doi:10.1016/S0079-6107(99)00010-3. PMID 10581972.

[pmid16669754-6] Jones-Rhoades MW, Bartel DP, Bartel B (2006). "MicroRNAS and their regulatory roles in plants". Annu Rev Plant Biol. 57: 19–53. doi:10.1146/annurev.arplant.57.032905.105218. PMID 16669754.

[7] Peng, Juan; Yu, Dashi; Wang, Liqun; Xie, Minmin; Yuan, Congying; Wang, Yu; Tang, Dongying; Zhao, Xiaoying; Liu, Xuanming (June 2012). "Arabidopsis F-box gene FOA1 involved in ABA signaling". Science China Life Sciences. 55 (6): 497–506. doi:10.1007/s11427-012-4332-9. ISSN 1869-1889. PMID 22744179.

[8] Ha, Van Den Burg; Tsitsigiannis, D. I.; Rowland, O; Lo, J; Rallapalli, G; Maclean, D; Takken, F. L.; Jones, J. D. (2008). "The F-box protein ACRE189/ACIF1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato". Plant Cell. 20 (3): 697.

[9] Moroishi, T; Nishiyama, M; Takeda, Y; Iwai, K; Nakayama, K. I. (2011). "The FBXL5-IRP2 axis is integral to control of iron metabolism in vivo". Cell Metabolism. 14 (3): 339.

[10] Kaiser, Peter; Su, Ning-Yuan; Yen, James L.; Ouni, Ikram; Flick, Karin (2006-08-08). "The yeast ubiquitin ligase SCFMet30: connecting environmental and intracellular conditions to cell division". Cell Division. 1: 16. doi:10.1186/1747-1028-1-16. ISSN 1747-1028.

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