English: Supplementary Movie 2 Three-dimensional reconstruction of an E. huxleyi cell from a cryo FIB-SEM

image series. Sequential images through a vitrified cell acquired with the energy selective backscattered detector, followed by sequential images through the same cell acquired with the In-lens secondary electron detector, followed by revealing the 3D segmentation of the plasma membrane (light green), the chloroplast (dark green), nucleus (violet), a coccolith in statu nascendi (blue), Ca-rich bodies (red) and the membranes (orange) encompassing the bodies. As the contrast in backscattered imaging correlates with the mean atomic number of the material, mature coccoliths (part of coccosphere) and the coccolith in statu nascendi (intracellular) as well as

the Ca-rich body appear bright. It corresponds to Figure 3.