STX10

STX10
Dostupne strukture
PDB	Pretraga Human UniProta: PDBe RCSB
Spisak PDB ID kodova
	4DND
Identifikatori
Aliasi	STX10
Vanjski ID-jevi	OMIM: 603765 HomoloGene: 84379 GeneCards: STX10
Lokacija gena (čovjek)
Hrom.	Hromosom 19 (čovjek)
Kraj	13,150,383 bp
Ontologija gena
Molekularna funkcija	• SNAP receptor activity; • syntaxin binding; • GO:0001948, GO:0016582 vezivanje za proteine; • SNARE binding;
Ćelijska komponenta	• integral component of membrane; • perinuklearno područje citoplazme; • Vezikula; • Golđijeva membrana; • trans-Golđijeva mreža; • Golđijev aparat; • SNARE complex; • membrana; • trans-Golgi network membrane; • citosol; • Sinapsna vezikula; • Endomembranski sistem; • integral component of synaptic vesicle membrane; • intracellular anatomical structure;
Biološki proces	• early endosome to Golgi transport; • protein transport; • vesicle docking; • intracellular protein transport; • Fuzija vezikula; • retrograde transport, endosome to Golgi; • Golgi vesicle transport; • vesicle-mediated transport; • regulation of protein localization; • membrane fusion;
	Izvori:Amigo / QuickGO
Ortolozi
	8677
	n/a
	ENSG00000104915
	n/a
	O60499
	n/a
	NM_001271609; NM_001271610; NM_001271611; NM_003765
	n/a
	NP_001258538; NP_001258539; NP_001258540; NP_003756
	n/a
	Wikipodaci
Pogledaj/uredi – čovjek

Sintaksin-10 (STX10) jest jedan od SNARE proteina, koji je kod ljudi kodiran genom STX10 sa hromosoma 19.^[3] Ovaj protein nalazi se kod većine kičmenjaka (uključujući ljude), ali ga primjetno nema kod miševa.^[4]^[5] Kao i kod ostaliih SNARE proteina, STX10 olakšava fuziju vezikula i stoga je važan za unutarćelijski promet proteina i drugih ćelijskih komponenti. Preciznije, STX10 je upleten u promet endosoma i Golgijevog aparata receptorom manoza 6-fosfata^[4] i transporterom glukoze tipa 4.^[5]

STX10 je otkriven u trans-Golgijevoj mreži (TGN) pomoću imunofluorescencije.^[3]

Aminokiselinska sekvenca uredi

Dužina polipeptidnog lanca je 249 aminokiselina, a molekulska težina 28.114 Da.^[3]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MSLEDPFFVV	RGEVQKAVNT	ARGLYQRWCE	LLQESAAVGR	EELDWTTNEL
RNGLRSIEWD	LEDLEETIGI	VEANPGKFKL	PAGDLQERKV	FVERMREAVQ
EMKDHMVSPT	AVAFLERNNR	EILAGKPAAQ	KSPSDLLDAS	AVSATSRYIE
EQQATQQLIM	DEQDQQLEMV	SGSIQVLKHM	SGRVGEELDE	QGIMLDAFAQ
EMDHTQSRMD	GVLRKLAKVS	HMTSDRRQWC	AIAVLVGVLL	LVLILLFSL

Struktura i funkcija uredi

Ljudski STX10 je protein od 249 aminokiselina koji ima tri N-terminalna α-heliksa i jedan SNARE domen nakon čega slijedi jednoprolazni transmembranski domen. Ljudski STX10 je 60% identičan ljudskom STX6.^[3]

STX10 je strukturno klasifikovan kao Qc-SNARE (doprinosi ostatku glutamina (Q) u formiranju sastavljenog jezgra SNARE kompleksa) i funkcionalno je klasifikovan kao t-SNARE (ili ciljni-SNARE koji se često nalazi u membranama ciljnih odjeljaka).^[6]

Interakcije uredi

Poznato je da STX10 komunicira sa t-SNARE-ovima VTI1A i STX16^[7] te sa v-SNARE VAMP3^[4] i VAMP4.^[7] SNARE kompleks STX10, STX16, VTI1A i VAMP3 je neophodan za kasni prenos endosoma ka Golgijevom manozni 6-fosfatni receptor.^[4] Rani promet endosoma ka Golgijevom aparatu za Shiga toksin potreban je SNARE kompleks STX6, STX16, VTI1A i VAMP3 ili VAMP4.^[4]^[8]

Dakle, STX10 diferencira rani promet endosoma u Golgijevom aparatu od kasnog prometa endosomom do Golgijeva kompleksa.^[4]

Reference uredi

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000104915 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b ^c ^d Tang BL, Low DY, Tan AE, Hong W (January 1998). "Syntaxin 10: a member of the syntaxin family localized to the trans-Golgi network". Biochemical and Biophysical Research Communications. 242 (2): 345–50. doi:10.1006/bbrc.1997.7966. PMID 9446797.
^ ^a ^b ^c ^d ^e ^f Ganley IG, Espinosa E, Pfeffer SR (January 2008). "A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells" (PDF). The Journal of Cell Biology. 180 (1): 159–72. doi:10.1083/jcb.200707136. PMC 2213607. PMID 18195106.
^ ^a ^b Esk C, Chen CY, Johannes L, Brodsky FM (January 2010). "The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step". The Journal of Cell Biology. 188 (1): 131–44. doi:10.1083/jcb.200908057. PMC 2812854. PMID 20065094.
^ Hong W (July 2005). "SNAREs and traffic". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1744 (3): 493–517. doi:10.1016/j.bbamcr.2005.03.014. PMID 16038056.
^ ^a ^b Wang Y, Tai G, Lu L, Johannes L, Hong W, Tang BL (Jul–Aug 2005). "Trans-Golgi network syntaxin 10 functions distinctly from syntaxins 6 and 16". Molecular Membrane Biology. 22 (4): 313–25. doi:10.1080/09687860500143829. PMID 16154903. S2CID 25014829.
^ Mallard F, Tang BL, Galli T, Tenza D, Saint-Pol A, Yue X, Antony C, Hong W, Goud B, Johannes L (February 2002). "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform". The Journal of Cell Biology. 156 (4): 653–64. doi:10.1083/jcb.200110081. PMC 2174079. PMID 11839770.

Vanjski linkovi uredi

PDBe-KB provides an overview of all the structure information available in the PDB for Human Syntaxin-10 (STX10)

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000104915 - Ensembl, maj 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[Tang_1998-3] Tang BL, Low DY, Tan AE, Hong W (January 1998). "Syntaxin 10: a member of the syntaxin family localized to the trans-Golgi network". Biochemical and Biophysical Research Communications. 242 (2): 345–50. doi:10.1006/bbrc.1997.7966. PMID 9446797.

[Ganley_2008-4] ^ ^a ^b ^c ^d ^e ^f Ganley IG, Espinosa E, Pfeffer SR (January 2008). "A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells" (PDF). The Journal of Cell Biology. 180 (1): 159–72. doi:10.1083/jcb.200707136. PMC 2213607. PMID 18195106.

[Esk_2010-5] Esk C, Chen CY, Johannes L, Brodsky FM (January 2010). "The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step". The Journal of Cell Biology. 188 (1): 131–44. doi:10.1083/jcb.200908057. PMC 2812854. PMID 20065094.

[Hong_2005-6] Hong W (July 2005). "SNAREs and traffic". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1744 (3): 493–517. doi:10.1016/j.bbamcr.2005.03.014. PMID 16038056.

[Wang_2005-7] Wang Y, Tai G, Lu L, Johannes L, Hong W, Tang BL (Jul–Aug 2005). "Trans-Golgi network syntaxin 10 functions distinctly from syntaxins 6 and 16". Molecular Membrane Biology. 22 (4): 313–25. doi:10.1080/09687860500143829. PMID 16154903. S2CID 25014829.

[Mallard_2002-8] Mallard F, Tang BL, Galli T, Tenza D, Saint-Pol A, Yue X, Antony C, Hong W, Goud B, Johannes L (February 2002). "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform". The Journal of Cell Biology. 156 (4): 653–64. doi:10.1083/jcb.200110081. PMC 2174079. PMID 11839770.

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