MT1X
Metalotionein 1X, znan i kao MT1X, je protein koji je kod ljudi kodiran genom MT1X.[3][4][5]Lokus zaporodicu gena MT1, koja uključuje MT1X, nalazi se na hromosomu 16, regija q13.
Aminokiselinska sekvenca uredi
Dužina polipeptidnog lanca je 61 aminokiselinu, a molekulska težina 6.068 Da.[6].
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MDPNCSCSPV | GSCACAGSCK | CKECKCTSCK | KSCCSCCPVG | CAKCAQGCIC | ||||
| KGTSDKCSCC | A |
Funkcija gena uredi
Prekomjerna ekspresija TCRP1 (FAM168A) promovira rezistenciju na cisplatin u oralnim pločastoćelijskim karcinomima (OSCC). Koristeći analizu mikromreža, Peng et al. (2012) identificirali su 30 gena koji su diferencijalno regulirani noktaundom TCRP1 u ćelijama OSCC. Gen koji je najznačajnije smanjen bio je MT1X. Kvantitativna rt-PCR analiza potvrdila je smanjenje regulacije MT1X nakon obaranja TCRP1. Eksperimenti koimunoprecipitacije i proteina otkrili su direktnu interakciju između TCRP1 i MT1X. Imunohistohemijska analiza 60 primarnih karcinoma pločastih usnih ćelija otkrila je pozitivnu korelaciju između ekspresije TCRP1 i MT1X i rezistencije na cisplatin. Nokdaun MT1X povećao je osetljivost ćelija na cisplatin i smanjio stvaranje kolonija nakon tretmana cisplatinom u ćelijama OSCC.[7][8][9]
Također pogledajte uredi
Reference uredi
- ^ a b c GRCh38: Ensembl release 89: ENSG00000187193 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: MT1X metallothionein 1X".
- ^ West AK, Stallings R, Hildebrand CE, Chiu R, Karin M, Richards RI (novembar 1990). "Human metallothionein genes: structure of the functional locus at 16q13". Genomics. 8 (3): 513–8. doi:10.1016/0888-7543(90)90038-V. PMID 2286373.
- ^ Stennard FA, Holloway AF, Hamilton J, West AK (august 1994). "Characterisation of six additional human metallothionein genes". Biochim. Biophys. Acta. 1218 (3): 357–65. doi:10.1016/0167-4781(94)90189-9. PMID 8049263.
- ^ "UniProt, P80297". Pristupljeno 8. 7. 2021.
- ^ Karin, M., Eddy, R. L., Henry, W. M., Haley, L. L., Byers, M. G., Shows, T. B. Human metallothionein genes are clustered on chromosome 16. Proc. Nat. Acad. Sci. 81: 5494-5498, 1984. PubMed: 6089206
- ^ Peng, B., Gu, Y., Xiong, Y., Zheng, G., He, Z. Microarray-assisted pathway analysis identifies MT1X & NF-kappa-B as mediators of TCRP1-associated resistance to cisplatin in oral squamous cell carcinoma. PLoS One 7: e51413, 2012. PubMed: 23251525
- ^ West, A. K., Stallings, R., Hildebrand, C. E., Chiu, R., Karin, M., Richards, R. I. Human metallothionein genes: structure of the functional locus at 16q13. Genomics 8: 513-518, 1990. PubMed: 2286373
Dopunska literatura uredi
- West AK, Stallings R, Hildebrand CE, et al. (1991). "Human metallothionein genes: structure of the functional locus at 16q13". Genomics. 8 (3): 513–8. doi:10.1016/0888-7543(90)90038-V. PMID 2286373.
- Stennard FA, Holloway AF, Hamilton J, West AK (1994). "Characterisation of six additional human metallothionein genes". Biochim. Biophys. Acta. 1218 (3): 357–65. doi:10.1016/0167-4781(94)90189-9. PMID 8049263.
- Pauwels M, van Weyenbergh J, Soumillion A, et al. (1994). "Induction by zinc of specific metallothionein isoforms in human monocytes". Eur. J. Biochem. 220 (1): 105–10. doi:10.1111/j.1432-1033.1994.tb18603.x. PMID 8119276.
- Mididoddi S, McGuirt JP, Sens MA, et al. (1996). "Isoform-specific expression of metallothionein mRNA in the developing and adult human kidney". Toxicol. Lett. 85 (1): 17–27. doi:10.1016/0378-4274(96)03632-6. PMID 8619255.
- Holloway AF, Stennard FA, West AK (1997). "Human metallothionein gene MT1L mRNA is present in several human tissues but is unlikely to produce a metallothionein protein". FEBS Lett. 404 (1): 41–4. doi:10.1016/S0014-5793(97)00082-3. PMID 9074634. S2CID 46527743.
- Garrett SH, Somji S, Todd JH, et al. (1999). "Differential expression of human metallothionein isoform I mRNA in human proximal tubule cells exposed to metals". Environ. Health Perspect. 106 (12): 825–32. doi:10.2307/3434126. JSTOR 3434126. PMC 1533231. PMID 9831543.
- Hellemans G, Soumillion A, Proost P, et al. (2000). "Metallothioneins in human kidneys and associated tumors". Nephron. 83 (4): 331–40. doi:10.1159/000045425. PMID 10575295. S2CID 26103380.
- Garrett SH, Sens MA, Shukla D, et al. (2000). "Metallothionein isoform 1 and 2 gene expression in the human prostate: downregulation of MT-1X in advanced prostate cancer". Prostate. 43 (2): 125–35. doi:10.1002/(SICI)1097-0045(20000501)43:2<125::AID-PROS7>3.0.CO;2-S. PMID 10754528.
- Vandeghinste N, Proost P, De Ley M (2000). "Metallothionein isoform gene expression in zinc-treated human peripheral blood lymphocytes". Cell. Mol. Biol. (Noisy-le-grand). 46 (2): 419–33. PMID 10774930.
- Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Rahman MT, De Ley M (2001). "Metallothionein isogene transcription in red blood cell precursors from human cord blood". Eur. J. Biochem. 268 (3): 849–56. doi:10.1046/j.1432-1327.2001.01947.x. PMID 11168427.
- Garrett SH, Belcastro M, Sens MA, et al. (2001). "Acute exposure to arsenite induces metallothionein isoform-specific gene expression in human proximal tubule cells". J. Toxicol. Environ. Health Part A. 64 (4): 343–55. doi:10.1080/152873901316981321. PMID 11693492. S2CID 43143430.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
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- Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.